EGFRVIII HEK293
CBP74691
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索取Protocol
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产品数据库
| I. Background | |
| EGFRvIII 是表皮生长因子受体（EGFR）最常见的胞外区突变体，由EGFR基因外显子 2-7 缺失导致，使其无法结合配体却具有持续激活的酪氨酸激酶活性。它是胶质母细胞瘤中常见的突变形式，在约 30% 的 GBM 中表达，与肿瘤增殖、侵袭及不良预后相关，且因正常组织不表达而成为极具吸引力的肿瘤特异性治疗靶点。 | |
| II. Description | |
| EGFRVIII HEK293 细胞，在 HEK293 细胞的基础上，稳定表达 EGFRVIII。 | |
| III. Introduction | |
| Expressed gene: | EGFRVIII |
| Host Cell: | HEK293 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10% FBS + 2 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Representative Data | |
 Figure 1. Recombinant EGFRVIII HEK293 constitutively expressing EGFRVIII. |
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